ELISA DNA Library Prep for Oxford Nanopore Technologies

VAHTS TGS DNA Library Prep Kit for Oxford Nanopore Technologies ONT Quantity: 24 Rxns. Keywords: DNA library construction; third generation library construction; TGS library construction; Nanopore sequencing; ONT sequencing VAHTS TGS DNA Library Prep Kit for ONT is a ligation-based library construction kit suitable for Nanopore sequencing platform. Combined with the Barcode Adapter, it can perform single-sample or mµLti-sample analysis of 100 ng - 1.5 μg gDNA and amplicons. Build a database. This kit uses a directed evolution mutant enzyme with high catalytic activity and a carefµLly optimized buffer. It can directly perform Barcode Ligation without purification after end repair/damage repair/A-tail addition, and the experimental process is convenient. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction. Features: 1) The operation process is simple: steps of end repair + damage repair + adding A tail are completed in one tube, no purification is required, and Barcode connection can be performed directly 2) The time required for library construction is shortened: the minimum time required for the entire process of database construction can be <2 hours, which saves ≥30 minutes compared with the conventional process. 3) Compatible with various species you need to work with: compatible with animals, plants, microorganisms and other species 4) Excellent data quality: high read rate of long fragments, high data output, and good data quality Better Data output and Quality Species Metric TS201 Supplier A Clean Total Reads 1,910,636 1,754,451 Clean Total bases 37,568,318,787 30,769,214,717 Clean Mean quality(Mean Q) 14.77 14.69 Plant Clean Total Reads 3,398,731 3,494,821 Clean Total bases 32,590,018,744 31,687,125,980 Clean Mean quality(Mean Q) 12.86 12.77 Bacteria Clean Total Reads 223,825 287,405 Clean Total bases 1,629,142,727 1,791,295,356 Clean Mean quality(Mean Q) 15.5 15.3 Better Longer Reads Species DNA Quantity TS201 Supplier A 5 - 50 kb 63.63% 60.95% 50-100 kb 8.25% 6.57% 100-200 kb 0.13% 0.11% Total 72.01% 67.63% Plant 5 - 50 kb 39.63% 37.02% 50-100 kb 2.22% 2.10% 100-200 kb 0.02% 0.02% Total 41.87% 39.14% Kit composition Component TS201-01 (24 rxns) End Prep & Repair Buffer 36 µL End Prep & Repair Master Enzyme Mix 36 µL Barcode Rapid Ligase Mix 96 µL MP Adapter Fast Ligation Buffer 240 µL MP Adapter Fast Ligase 120 µL