bHB ELISA

bHB (Beta-Hydroxybutyric Acid) ELISA This assay employs the competitive inhibition enzyme immunoassay technique. The microplate provided in this kit has been pre-coated with Beta-Hydroxybutyric Acid (bHB) protein. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Beta-Hydroxybutyric Acid (bHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Beta-Hydroxybutyric Acid (bHB) in the samples is then determined by comparing the OD of the samples to the standard curve. Kit Components: 1) Pre-coated Microplate [12 strips x 8 wells] 2) 100x Biotinylated Conjugate [120ul] 3) Biotinylated Conjugate Diluent [10ml] 4) 100x Streptavidin-HRP [120ul] 5) HRP Diluent [12ml] 6) TMB Substrate Solution [9ml] 7) 25x Wash Buffer [20ml] 8) Stop reagent [6ml] 9) Lyophilized Standard [2] 10) Standard Diluent Buffer [20ml] 11) Plate Sealers [2] Intend use : • 5% GST extra on Product • Packaging, Handling & Transport charges (with GST) extra as applicable. • Delivery period : 3-4 weeks or earlier as per availability.