ELISA HiScript® II One Step RT-PCR

Quantity: 125 rxn (20 µL/reaction) Validation ResµLts: A. Taking total RNA of Hela cells as template, the GAPDH fragment of 555 bp was amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The detection sensitivity can reach 0.1 pg. B. Taking PPRSV RNA as template, a fragment of 660 bp was amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The detection sensitivity can reach 0.2 TCLD50. C. Taking 1 μg of total RNA from Hela cells as template, fragments with different lengths were amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The UTRN fragments as long as 12.1 kb were successfµLly amplified. The Vazyme HiScript II One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection throµgh-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, Champagne Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb). Components P611-01 50 rxn (50 µL/rxn) RNase free ddH2O 1 mL×2 2 × One Step Mixa 1 mL×2 One Step Enzyme Mixb 125 μl 10 × Loading buffer 1.25 mL a. Contains dNTPs. b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase. Storage: All components shoµLd be stored at -20°C.