Methylamp RNA Bisulfite Conversion Kit

A complete set of optimized reagents required for fast bisulfite conversion on a RNA sample. 50 reactions. The converted RNA obtained with the Methylamp RNA Bisulfite Conversion Kit is suitable for various downstream RNA methylation analyses including methylation specific RT-PCR, MS-HRM, and bisulfite RNA-sequencing (e.g., pyrosequencing and deep-sequencing). • • Fast and convenient protocol that can be finished in 3 hours. • Completely converts unmethylated RNA cytosine into uracil (>99.9%) with no or negligible inappropriate/error conversion of methylcytosine to thymine (<0.1%) when the indicated range of input sample RNA is used. • Powerful protection against RNA degradation, with over 90% of RNA loss prevented. • Included control primers are specific against bisulfite-converted RNA and can be used to test whether the bisulfite conversion has been properly achieved. • Low amount of input RNA can be used for bisulfite conversion with as low as 5 ng per reaction. • Simple, reliable, and consistent reaction conditions.