ChromaFlash High Sensitivity ChIP Kit (48 reactions)

A complete set of optimized reagents to carry out a successful chromatin immunoprecipitation procedure in a high throughput format starting from mammalian cells or tissues. The highly specific and sensitive kit is suitable for selective enrichment of a chromatin fraction containing specific DNA sequences using various mammalian cell/tissues. The optimized protocol and kit components reduce non-specific background ChIP levels to allow capture of low abundance protein/transcription factors and increased specific enrichment of target protein/DNA complexes. The target protein bound DNA prepared with the ChromaFlash High-Sensitivity ChIP Kit can be used for various downstream applications including PCR (ChIP-PCR), microarrays (ChIP-on-chip), and sequencing (ChIP-seq). The kit has the following advantages: • The highly efficient enrichment ratio of positive to negative control is > 500. An extremely low number of cells (as low as 2,000 cells per ChIP reaction) can be used for enriching highly abundant protein/DNA complexes. • optimized buffers and protocol allow minimal ChIP background by overcoming the weaknesses that cause non-specific enrichment, thereby increasing sensitivity and specificity of the ChIP reaction. • Increased antibody selectivity and capture efficiency through the use of unique chimeric proteins containing the maximum number of IgG binding domains coated on the strip-wells. This allows strong binding of any IgG subtype antibodies within a wide pH range regardless if they are in monoclonal or polyclonal form. • Fast 5 hour procedure, from input chromatin to ready-to-use DNA eluate. • 96-well plate format makes the assay flexible. Either (a) manual with one single reaction each time; or (b) high throughput with 24-48 reactions each time. • Wide downstream analysis compatibility. Compatible with various downstream analysis workflows including ChIP-PCR, ChIP-on-chip, and ChIP-seq.