ELISA Acetyl-NF-kappaB p65 (Lys310) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:RELA GeneID:5970 Uniprot:Q04206 Specificity:Acetyl-NF-kappaB p65 (Lys310) Antibody detects endogenous levels of Acetyl-NF-kappaB p65 only when acetylated at Lys310. Immunogen:A syntheQuantityd peptide derived from NF-kappaB p65 around the acetylation site of Lys310. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl. Reactivity:,Mouse,Rat Prediction:Pig,Bovine,Horse,Sheep,Dog Application:WB,IHC,IF/ICC,ELISA(peptide) Dilution:WB: 1:500-1:2000, IHC: 1:50-1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2835385 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF1017-BP Des# Blocking Peptide:Acetyl-NF-kappaB p65 (Lys310) Blocking peptide Blocking Peptide Price:$549 CAD