ELISA Rat Plasma kallikrein (KLKB1)

Reactivity:Rat (Rattus norvegicus) UniProt:P14272 Abbreviation:KLKB1 Alternative Names:KLK3; PPK; Fletcher factor|kininogenin|plasma kallikrein B1|plasma kallikrein heavy chain|plasma kallikrein light chain Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.063 ng/mL Intra-AssayCV:?4.9% Inter-AssayCV:?10.8% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KLKB1 in samples. An antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KLKB1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: prostate-specific antigen (APS) is a kallikrein-like protease present in seminal plasma. It is a single-chain glycoprotein with a molecµLar mass of about 33 kD that may function normally in the liquefaction of seminal coagµLum. Radioimmunoassay of serum levels of this antigen (called PSA in the clinical setting) is usefµL in the diagnosis and monitoring of prostatic carcinoma. The fµLl-length KLK3 protein contains 261 amino acids. It has a putative signal peptide, followed by a short activating peptide and the protease domain, which includes the catalytic triad of his65, asp120, and ser213. RT-PCR of 35 adµLt and fetal tissues detected highest expression in prostate. Expression was weak in colon, mammary gland, and parotid, and little to no expression was detected in other tissues examined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).