ELISA 7SK snRNA methylphosphate capping enzyme (MEPCE)

Reactivity: (Homo sapiens) UniProt:Q7L2J0 Abbreviation:MEPCE Alternative Names:BCDIN3; FLJ20257; 7SK snRNA methylphosphate capping enzyme|bin3; bicoid-interacting 3; homolog Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?3.6% Inter-AssayCV:?7.6% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MEPCE in samples. An antibody specific for MEPCE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEPCE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MEPCE is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPCE bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).