ELISA 8-Hydroxy-desoxyguanosine (8-OHdG)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:8-OHdG Alternative Names:N/A Application:ELISA Range:12.5-800 ng/mL Sensitivity:6.0 ng/mL Intra-AssayCV:?3.9% Inter-AssayCV:?7.5% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate 8-OHdG in samples. An antibody specific for 8-OHdG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any8-OHdG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for 8-OHdG is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 8-OHdG bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:8-oxo-7,8-dihydro-2 deoxyguanosine(8-OHdG), is probably the most important product of "oxidative stress” in DNA. Its concentration in DNA is, in fact. a quantitative analysis of the degree of DNA damage that an organism has undergone. Due to the importance of 8-OHdG of nucleic acidg in mutagenesis, carcinogenesis and aging, numerous chemical and biological investigations have been made on this subject in the past time. Kuchino and co-workers have found that 8-OHdG residue in DNA is misreading during the process of DNA replication. Recently, some reports have been presented on high 8-OHdG levels in patients suffering from various diseases such as chronic hepatitis, Fanconi s anemia, diabetes mellitus and Helicobacter pylori infections. As a resµLt, 8-OHdG is a usefµL marker for the study of DNA damage arising from reactive oxygen species and is of great significance for cancer research. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).