ELISA 17-ketosteroids (17-KS)
Reactivity: (Homo sapiens)
UniProt:N/A
Abbreviation:17-KS
Alternative Names:N/A
Application:ELISA
Range:3.125-200 ng/mL
Sensitivity:1.25 ng/mL
Intra-AssayCV:?7.1%
Inter-AssayCV:?8.5%
Recovery:0.96
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate 17-KS in samples. An antibody specific for 17-KS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any17-KS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for 17-KS is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 17-KS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:A 17-ketosteroid is a steroid that has a ketone (C=O) functional group at the C17 position (in the upper right corner of most diagrams.) These compound are products of metabolization of testosterone in most tissues such as liver.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
This kit is an essential tool for professionals seeking to conduct detailed analyses of neurotransmitter levels in urine, aiding in the understanding and management of various neurological and psychiatric conditions.
Internal Reference:
AE22896HU
Website URL:
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