ELISA 12-hydroxyeicosatetraenoic acid (12-HETE)
Reactivity: (Homo sapiens)
UniProt:N/A
Abbreviation:12-HETE
Alternative Names:N/A
Application:ELISA
Range:12.35-1000 ng/mL
Sensitivity:4.64 ng/mL
Intra-AssayCV:?4.1%
Inter-AssayCV:?7.6%
Recovery:0.94
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate 12-HETE in samples. An antibody specific for 12-HETE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any12-HETE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for 12-HETE is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 12-HETE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:12-Hydroxyeicosatetraenoic acid (12-HETE) is an eicosanoid. It is a metabolite of arachidonic acid produced by the action of the enzyme arachidonate 12-lipoxygenase. 12-HETE is a highly selective ligand used to label mu opioid receptors. 12-HETE can activate Cdc42, Rac1 and PI-3K, which then participate as upstream signalling molecµLes for PAK1 and JNK1 activation. 12-Hydroxyeicosatetraenoic acid (12-HETE) is a chemotactic stimµLus for epidermal cells. 12-hydroxyeicosatetraenoic acid (12-HETE), the main eicosanoid in skin, is assumed to have both pathophysiologic effects in inflammatory skin diseases such as psoriasis and atopic eczema and a physiologic role in the biology of cutaneous reparative processes. The modµLation of epidermal 12-HETE receptors by UV-B may partly explain the therapeutic effects of UV-B, but possibly also contribute to photodamage to skin.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
This kit is an essential tool for professionals seeking to conduct detailed analyses of neurotransmitter levels in urine, aiding in the understanding and management of various neurological and psychiatric conditions.
Internal Reference:
AE22885HU
Website URL:
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