GelRed Nucleic Acid Stain 10,000X in water - 0.5 ml

High-sensitivity nucleic acid stain for gel electrophoresis, providing clear, sharp bands, supplied by Gentaur's warehouse.

GelRed™ Staining Protocol (Precast Method)

Product: GelRed™ Nucleic Acid Stain, 10,000X in water

Volume: 0.5 ml (sufficient for 100+ gels depending on usage)

Applications: dsDNA, ssDNA, RNA visualization

 I. Materials Required

Agarose

TAE or TBE buffer

DNA samples + loading dye

GelRed™ 10,000X in water

Gel casting tray and combs

Microwave or hot plate

Electrophoresis apparatus

UV or Blue LED transilluminator

🧫 II. Precasting Gel with GelRed™

This method avoids post-staining and reduces handling time.

1. Prepare Agarose Gel:

Dissolve agarose in 1X TAE or TBE buffer (e.g., 1 g agarose in 100 mL buffer for 1% gel).

Heat until completely melted (microwave or hot plate).

2. Cool Gel:

Let the gel solution cool to about 60°C (touch warm, not hot).

3. Add GelRed™:

Add 5 µL of 10,000X GelRed™ to 50 mL of molten agarose.

Mix gently (avoid bubbles).

✅ Dilution Factor: 1:10,000

 Do not add GelRed™ to very hot agarose — may degrade stain.

4. Cast the Gel:

Pour into tray, insert combs, and allow to solidify (~30 minutes).

5. Run the Gel:

Load samples and run electrophoresis as usual.

6. Visualize DNA:

Use UV or blue light transilluminator (blue preferred to reduce DNA damage).

Use orange filter if imaging with blue light.

🧪 III. Post-Staining Method (if desired)

If not precast, you can post-stain:

1. Dilute GelRed™:

Add 5 µL of 10,000X GelRed™ to 50 mL water or buffer.

2. Stain Gel:

Submerge gel in staining solution.

Incubate 30 minutes with gentle agitation (light-protected).

🔐 Storage & Safety

Store at room temp (RT) or 4°C, protected from light.

Non-mutagenic, safer than ethidium bromide (see Biotium's safety data).

Dispose of according to institutional biosafety guidelines.